Fig. 4

Morphological analysis of the Keio collection reveals correlations between morphological features but not with growth rate. Contours from >150 cells per Keio deletion strain were extracted from images acquired from the NBRP repository and used to compute the mean length and width and standard deviation of cell width across each population. In (a-d), white circles and error bars were obtained by binning strains by mean width or length; blue lines are the fit to binned averages. R is Pearson’s correlation coefficient. a-b Heatmaps of the number of strains with geometry parameters in each bin show that mean length (a) and standard deviation of mean width (b) are positively correlated with mean width. c-d Neither mean width (c) nor mean length (d) are correlated with maximal growth rate, as measured from microplate growth curves in [41]. e-h Top: Representations of the PCA modes around the mean shape. Modes 1–4 represent elongation, bending, width, and tapering, respectively. Bottom: scatter plots of the proportion of modes 1–4 and mean cell width of each strain demonstrate that width is correlated with variation represented by modes 1, 3, and 4