Fig. 6

P. infestans shows increased biomass on cyp79b2/b3 compared to Col-0 in the absence of Albugo, but not in its presence. a P. infestans biomass on mutants in the tryptophan-derived metabolite pathway. Leaves were inoculated with 100 μL of 1 × 10⁵ spores per mL P. infestans 88069td. DNA was extracted at 3 dpi and the proportion of P. infestans DNA to plant DNA determined using qRT-PCR. Open circles and bars denote the mean ± SE of P. infestans DNA (log₂ transformed normalized relative quantities (NRQs)) in Arabidopsis tissue from four independent biological replicates with three technical replicates per biological replicate. Closed, black circles denote the individual data points. Different letters indicate significant differences (P < 0.01) (Kruskal–Wallis rank sum test with Dunn multiple comparisons test and Bonferroni correction). b Higher P. infestans biomass on AlNc14-infected Arabidopsis than on cyp79b2/b3. Leaves were inoculated with 100 μL of 1 × 10⁵ spores per mL P. infestans 88069td. DNA was extracted at 3 dpi and the proportion of P. infestans DNA to plant DNA determined using qRT-PCR. Open circles and bars denote the mean ± SE of P. infestans DNA (log₂ transformed NRQs) in Arabidopsis tissue from three independent biological replicates with three technical replicates per biological replicate. Closed, black circles denote the individual data points. Different letters indicate significant differences (P < 0.01) (Two-way ANOVA with Tukey’s honest significance difference test)