Fig. 5

NPFR in ab3A neurons modulates olfactory-guided behavior. a Photo and schematic of the single fly olfactory trap assay. b Ethyl butyrate (10⁻⁴ v/v) and fresh apple juice (10⁻¹ v/v) induce very similar electrophysiological responses in the OSNs of the large antennal basiconic sensilla ab1–ab3. Odor-evoked responses are presented as means ± standard error. Replicate numbers for EB - ab1A/B = 7; EB - ab2A = 7; EB - ab2B = 7; EB - ab3A = 9; EB - ab3B = 6; Apple - ab1A/B = 8; Apple - ab2A = 8; Apple - ab2B = 8; Apple - ab3A = 8; Apple - ab3B = 8. c Control experiments validating the single fly olfactory trap assay. w ¹¹¹⁸ flies are robustly attracted to fresh apple juice, but not to water. Orco ¹ mutants, which have defective OR trafficking and severely disrupted odor detection, show only slightly higher attraction to the apple juice bait (A) than w ¹¹¹⁸ flies show to water (W). Ab3A neuron-specific knock-down of Or22a disrupts attraction to apple juice, as Or22a > Or22a-IR flies are much less successful finding the bait than heterozygous controls (crossed to w ¹¹¹⁸). Replicate numbers for w ¹¹¹⁸ W to A = 39; w ¹¹¹⁸ A to W = 39; w ¹¹¹⁸ W to W = 39; Orco ¹ W to A = 26; Or22a-GAL4/+ W to A = 30; UAS-Or22a-IR/+ W to A = 30; Or22a > Or22a-IR W to A = 30. d Knock-down of NPFR in Orco-positive neurons (Orco-Gal4) and in ab3A neurons (Or22a-Gal4) significantly reduces attraction to apple juice. The data in c and d are presented as the percentage of flies reaching the bait (i.e., number of successes/number of single fly assays); P values were determined with Fisher’s exact test, P ≤ 0.05 (*), P ≤ 0.01 (**), P ≤ 0.001 (***). Replicate numbers for Orco > NPFR-IR = 20; Orco-GAL4/+ = 20; UAS-NPFR-IR/+ = 64; Or22a-GAL4/+ = 43; Or22a > NPFR-IR = 43