Fig. 6
REX2 and REX3 increase N. benthamiana susceptibility to P. palmivora, and REX3 interferes with host secretion. Transgenic N. benthamiana plants expressing GFP16c (control) or GFP:FLAG-REX1 to GFP:FLAG-REX4 were challenged with zoospores from P. palmivora YKDEL, and disease progression was ranked over time using the previously defined symptom extent stages (SESs). a Representative disease progression curves for transgenic plants expressing GFP:FLAG-REX1 (yellow), GFP:FLAG-REX2 (blue), GFP:FLAG-REX3 (green) or GFP:FLAG-REX4 (magenta) compared to GFP16c control plants (red dashed). p values were determined based on Scheirer-Ray-Hare nonparametric two-way analysis of variance (ANOVA) for ranked data. The experiment was carried out in duplicate (N = 22 plants). b Representative pictures of infected plants, 8 days after infection. c Disease-promoting effectors REX2 and REX3 were co-expressed with a secreted GFP construct (SPPR1-GFP) in N. benthamiana leaves. GFP fluorescence was quantified along the nucleus