Table 3 Buffer recipes

IHC blocking buffer

PBS with 0.5% BSA (Calbiochem) and 0.3% Triton X-100 (Sigma)

IHC washing buffer

PBS with 0.2% BSA (Calbiochem) and 0.1% Triton X-100 (Sigma)

Digestion buffer

HBSS (Corning) with 2% FBS (Gemini), 10 mM HEPES (Corning) and 2 mM CaCl₂ (Sigma)

FACS buffer

PBS (Lonza) with 5% FCS (Invitrogen-Gibco) and 5 mM EDTA (Boston BioProducts)