Fig. 1

Mov10 is significantly elevated in young mouse brain and is both nuclear and cytoplasmic. a Brain extract (25 μg) from C57BL/6 at ages indicated was immunoblotted for Mov10 and eIF5α as a loading control (top panel). Bar graph of the three independent experiments is shown in the bottom panel. Spearman’s rank-order correlation (ρ (70) = –0.371, ***p = 0.001). b and c 3,3′-Diaminobenzidine (DAB) stain of P1 brain (sagittal section) counterstained with hematoxylin. CTX cortex, HC hippocampus, CB cerebellum, HB hindbrain, MB midbrain. b No primary antibody; c Mov10 antibody. Images obtained using the Hamamatsu NanoZoomer slide scanning system. Scale bar = 1 mm. d–i Mov10 immunohistochemistry of P2 brain (d–f) and adult hippocampus (g–i). Scale bar = 10 μm