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Fig. 4 | BMC Biology

Fig. 4

From: Mov10 suppresses retroelements and regulates neuronal development and function in the developing brain

Fig. 4

Mov10 is required for neurite outgrowth and neuronal morphology. ac Brightfield images of N2a (WT), Mov10 knockout N2a (KO), and Mov10 transgene rescue of KO (Rescue). Scale bar represents 200 μm. d Quantification of neurite length of WT, KO, and Rescue, analyzed by one-way analysis of variance (ANOVA) (F (2,19) = 32, p = 0.000, p values, ** 0.01, *** 1.60484E-06). Error bars represent SEM. There were 500–800 differentiated neurons counted from triplicate experiments, and a total of 11 images were counted per condition. Lower panel is Mov10 immunoblot of WT, KO, and Rescue; eIF5α is loading control. e Schematic of significantly changed genes between WT undifferentiated and differentiated N2a. The number of differentially expressed genes as determined by Cuffdiff (p value <0.005 and fragments per kilobase million, FPKM >1) under both conditions is displayed (top). Venn diagram of genes differentially expressed in the KO versus WT. Orange (813): genes identified from comparison between undifferentiated WT and KO; green (781): genes identified from comparison between differentiated WT and KO; purple (513): Mov10-regulated genes (bottom). f Enrichment map of top Gene Ontology terms for the 513 Mov10-regulated genes (DAVID, p value <0.025) showing enrichment for genes related to nervous system development, axon guidance, and neuron projection. Fraction of genes up- (blue) and down- (orange) regulated in KO cells. g Microtubule-associated protein 1b (Map1b) immunoblot from WT, Mov10 KO, and rescue. Gapdh is the loading control. h Significantly changed genes between WT differentiated and undifferentiated (324). Of those genes, 64 significantly changed in the opposite direction of WT in the KO

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