Fig. 4.

Spectral imaging reveals organelle interactions. a Left: micrograph of a COS-7 fibroblast cell expressing fluorescent fusion proteins LAMP1-CFP, mito-EGFP, ss-YFP-KDEL, mOrange2-SKL, and mApple-SiT, and labeled with BODIPY 665/676. Images were acquired on a confocal microscope with a spectral detector, and subjected to linear unmixing and spatial deconvolution. Scale bar, 10 μm. Right: enlargement of the region outlined in the left panel. Scale bar, 5 μm. b Examples of complex organelle contacts in segmented multispectral lattice light-sheet images. The ER (transparent yellow) is shown in the right panels only. Scale bar, 2 μm