Fig. 5.

Comparison of saturated vs targeted annotation of three-dimensional EM data sets. The images have not been previously published as such, but are accessible from the datasets [32] published in [13]. a Electron micrograph of mouse lateral geniculate nucleus. b Saturated annotation of cell identities. Each cell profile from the electron micrograph in panel a is assigned a different color. c Examples of ultrastructural annotation that form additional layers of information in a saturated annotation of EM volume. Red = nucleus, yellow = glial encapsulation of synaptic glomerulus, green = distinctly light mitochondria of retinal ganglion cells, blue = synaptic sites between retinal ganglion cell boutons and thalamocortical neurons, and cyan = myelination of axons. d Synaptically targeted annotation of three-dimensional EM volume in which seed thalamocortical cell is green, the retinal ganglion cells that innervate the seed cell are red, and the thalamocortical cells that share retinal ganglion cell innervation with the seed cell are blue