Skip to main content

Table 1 Overview of internally tagged protein variants

From: Sequence-based prediction of permissive stretches for internal protein tagging and knockdown

  Stretch Span Insertion site Original sequencea Sequence after insertionb
Plasmid insertions
 Adk PSI I72-R78 D76 QEDCRNGFLLD 1-QEDENLYFQGLLD
PSII D94-A95 A93 PQADAMKE PQAENLYFQGMKE
K97 AMKEAG AMKENLYFQGEAG
A99 KEAGIN 1-KEAENLYFQGMKEAGIN
2-KEAENLYFQGDAMKEAGIN
PSIII V142-G150 P140 NPPKVEGKDDVTGE NPPENLYFQGTGE
PSIV T191-P201 A186 KEAEAGNTK KEAENLYFQGNTK
 GpsA PSI P55-V57 C49 DRCNAAFLPDVPFPD DRCENLYFQGFPD
P60 PFPDTL PFPENLYFQGVPFPDTL
PSII P97-D102 M99 PLMRPD PLMPTTENLYFQGCLGRPD
PSIII L128-Q131 I132 DQIPLA DQIPTTENLYFQGCLGPLA
PSV D272-V273 Q269 LGQGMD LGQPTTENLYFQGGTVGMD
 TpiA PSIII E53-I59 E55 EAEGSH 1-EAEGGSGENLYFQ G SGGSGSH
2-EAEGCLGESENLYFQGDERKNKGSH
3-EAEGCLPTTENLYFQSGTVKNKGSH
PSIV D67-N69 N69 DLNLSG DLNENLYFQGLSG
PSVI E133-A156 T130 GETEAENEAGKTE 1-GETENLYFQGGSGKTE
2-GETGGSENLYFQGGSGKTE
T153 LKTQGA LKTDYDIPTTENLYFQSGTVDAGADQGA
Chromosomal insertions
 Adk PSI I72-R78 D76 QEDCRN 3-QEDENLYFQGESLFKCRN
 TpiA PSIV D67-N69 L70 LNLSGA LNLPPKNENLYFQGESLFKGPSGA
 AtpA PSIII H123-F126 H123 LDHDGE LDHENLYFQGDGE
 AtpD PSIII E101-E105 E101 KGEIGE KGEENLYFQGIGE
Extended TEV-tag
 Adk PSI I72-R78 D76 QEDCRN 2-QEDENLYFQGCRN
     4-QEDENLYFQGESLFKGGCRN
 GpsA PSI P55-V57 D56 LPDVPS 1-LPD ENLYFQG VPS
     2-LPD PPKNENLYFQGESLFKGPVPS
  1. aResidues deleted during the insertion process are shown in boldface. If no residues were deleted, a 6-residue stretch of the protein sequence is shown, and the insert was placed in the middle
  2. bMinimal TEV-tag and extended derivatives used for insertion are shown in boldface