Fig. 8

Functional enrichment analysis of the most overexpressed genes of both cell types. The 100 most overexpressed genes of each cell type were classified based on Pfam, GO, and PANTHER assignments according to gene annotation v2.1 on the Phytozome V12 platform. Because many genes came without functional gene annotation, those genes were subject to individual BLASTP searches and the annotation of the hit with the highest sequence similarity was used for classification. Nevertheless, genes without any BLASTP-hits remained and therefore had to be excluded from this analysis due to the missing functional classification. All other involved genes obtained a functional assignment. Functional groups that contained not more than one member in both cell types were combined in the group ‘other functions’ for reasons of clarity. a Classification of the most overexpressed genes in somatic cells. b Classification of the most overexpressed genes in reproductive cells