Fig. 8
From: De novo assembly of the complex genome of Nippostrongylus brasiliensis using MinION long reads
Repeat sequence confounds Trinity gene prediction. a CAZy analysis indicated that two adjacent Trinity-generated transcripts (c16_g1_i2 and c16_g1_i1, left and right, respectively; exons indicated by blue boxes) had been incorrectly predicted since they correspond to C- and N-terminal fragments of a single CAZyme gene (of the GT33 family). The read coverage of the exons for the two predicted transcripts was similar, supporting a single-gene model. BUSCO erroneously called c16_g1_i1 as complete (EOG091H03EM0). b A self-map dot plot of the genomic context in which these transcripts reside demonstrated a high degree of sequence similarity throughout the region, where the two predicted transcripts were split by a palindromic repeat motif that switched directions at the intersection of the two transcripts. The coordinates (in kbp) on the contig tig000206 are shown