Fig. 5

Quantifying intracellular fluorescent protein aggregation. a Representative images of polyQ35-YFP-expressing animals at days 2, 4, and 6 of adulthood. BF bright-field illumination. Fluorescent protein aggregations are marked with white arrows. Scale bars = 100 μm. b Quantification of polyQ35-YFP puncta at days 2 (red, n = 64), 4 (blue, n = 37), and 6 (green, n = 42) of adulthood. Each dot represents an individual worm. Bars represent mean ± standard deviation (****p < 10^–4, one-way ANOVA test with Bonferroni post hoc correction). c Quantification of the relative sizes of polyQ35-YFP puncta on days 2 (red), 4 (blue), and 6 (green) of adulthood. Values for mean puncta size per worm as percent of worm size were log (base e) transformed. Each dot represents an individual worm. Bars represent mean ± standard deviation (***p < 0.001, *p < 0.05, one-way ANOVA test with Bonferroni post hoc correction)