Fig. 8

Phenotypic versus genotypic sex of sox5-/-mutants and regulation of the dmrt1 co-orthologs and a set of germ-cell markers in sox5-/- mutant embryos. a Phenotypic versus genotypic sex of sox5^-/- mutant fish. Complete XX female-to-male sex reversion was obtained. b Sexual phenotype of the adult medaka. Wild-type XX females have a triangular-shaped anal fin as well as fused dorsal fin rays. Wild-type XY males have a parallelogram-shaped anal fin as well as split dorsal fin rays. c Regulation of the expression of dmrt1bY and other germ-cell markers (nanos2, nanos3, dead-end, vasa, tra2a, and piwi) in sox5^-/- mutants compared to wild-type embryos at different stages of development (stages 28, 32, and 39). Dataset results of three different batches of eggs obtained from different couples. Statistical significance was assessed with the t-test (N = 3 and each replicate is a pool of 25 eggs; * p value ≤ 0.05, ** p value ≤ 0.01). ns non-significant