Fig. 5
From: A phosphoinositide map at the shoot apical meristem in Arabidopsis thaliana
Induction of PIP2 after mechanical perturbations. a NPA-treated seedlings were transferred to a medium without NPA 1 day before t = 0 h. At t = 0 h, a small ablation was performed with a needle and the resulting impact on PIP2 biosensor signal intensity was recorded at t = 24 h. b NPA-treated seedlings were transferred to a medium without NPA 1 day before t = 0 h. At t = 0 h, whole plants were immersed in 30 μM isoxaben and the resulting impact on PIP2 biosensor signal intensity was recorded at t = 24 h. c Correlation between cell size and signal intensity with or without isoxaben treatment. Gaussian curvature (central panel, unit: 10-3 μm-2) and signal intensity (right panel, %) were extracted as in other figures. Scale bar, 20 μm