Fig. 7
Open source segmentation protocol (CellProfiler). a Comparison of the image segmentation output for phb-2(tm2998) mutants generated from Developer Toolbox (GE Healthcare) versus CellProfiler. b Nile Red staining of phb-2(tm2998) mutants versus wild-type animals. Representative images taken with the IN Cell Analyzer and graphical representation of data coming from the different segmentation protocols. As previously shown [47], depletion of phb-2 reduces Nile Red staining using both of the protocols. (Mean±SD; ***P value < 0.0001 (Developer Toolbox) and ***P value = 0.0008 (CellProfiler); two-tailed unpaired t test; n > 45 for both conditions, combination of 3–5 different wells.) c Comparison of the UPRmt reporter signal after image analysis from Developer Toolbox (GE Heathcare) versus CellProfiler. The box plots represent fold change (FC) mean intensities of the RNAi clones from a randomly chosen 96-well plate from the UPRmt RNAi screen. RNAi clones with a P value < 0.001 appear in yellow and candidates with a P value < 0.001 and an FC < 0.5 appear in dark green. Control wells (control RNAi) appear in grey and positive control (atfs-1(RNAi)) in black. By comparing both box plots, we see that results from the different segmentation protocol are highly similar