Factors driving metabolic diversity in the budding yeast subphylum

Table 1 Utilization of diverse carbon sources is negatively associated with growth at high temperatures

Utilization	Observed	Expected	p _adj	Difference
Sucrose	428.000	374.15	<0.0001	53.8478
Galactose	445.000	396.3	<0.0001	48.7038
Trehalose	418.000	377.93	0.007	40.0716
Maltose	424.000	366.55	<0.0001	57.4544
Melezitose	402.000	352.02	<0.0001	49.9786
Methyl-α-D-glucoside	386.000	340.78	<0.0001	45.2166
Cellobiose	416.000	370.55	<0.0001	45.4488
Salicin	416.000	371.1	<0.0001	44.896
L-sorbose	429.000	382.19	<0.0001	46.8062
D-xylose	412.000	375.23	0.010	36.769
Ribitol	430.000	375.53	<0.0001	54.4678
D-mannitol	438.000	399.61	<0.0001	38.386
D-glucitol	447.000	404.41	<0.0001	42.5884
D-glucosamine	402.000	354.46	<0.0001	47.5448
2-keto-D-gluconate	413.000	363.27	0.002	49.7272

The observed data are a count of when one trait (e.g., the carbon utilization trait or growth at 37 °C) is present, while the other trait is absent. The expected value is the average count of the presence of either the carbon utilization trait of interest or growth at 37 °C and the absence of the other trait across 10,000 permutations. The difference is the observed minus the expected columns. We corrected for multiple tests across associations with the Benjamini–Hochberg correction (q < 0.05 shown, all data in Additional file 2: Table S2), which is shown in the column p_adj

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