Fig. 4
From: Downregulation of the NLRP3 inflammasome by adiponectin rescues Duchenne muscular dystrophy
Effects of ApN cDNA and pre-miR-711 electrotransfer on NLRP3 in skeletal muscles of ApN-KO mice. One tibialis anterior muscle of ApN-KO mice was injected and electroporated with ApN cDNA-containing plasmid (p-ApN) or with pre-miR-711-containing plasmid (p-miR-711), whereas the contralateral muscle was injected and electroporated with the respective control plasmid (p-ctrl). Nine days later, mice were challenged by LPS and the tibialis anterior muscles were sampled 24 h later. a Immunochemistry was performed with a specific antibody directed against NLRP3. Scale bar = 50 Ī¼m. b Quantification of 3,3ā-diaminobenzadine (DAB) staining areas within muscles. Results are means Ā± SEM for two groups of five mice (the contralateral muscle being used as control). *P < 0.05 for the effect of ApN or miR-711