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Fig. 6 | BMC Biology

Fig. 6

From: Harnessing single-cell genomics to improve the physiological fidelity of organoid-derived cell types

Fig. 6

CI-PCs reveal putative function of Nupr1 transcription factor in Paneth cell (PC) survival. a ENR + CD is enriched for in vivo PC and EEC transcription factors, including Nupr1 (n = 4). b Violin plots for the count-based expression level (log(scaled UMI + 1)) of Nupr1 across in vivo and in vitro called clusters. c Nupr1 inhibition with trifluorperazine (TFP) treatment concurrent with 6-day ENR + CD differentiation reveals dose-dependent toxicity, with preference to PCs (CD24+ and LYZ+) and PC-like (CD24+ and LYZ+) populations as assessed by flow cytometry (n = 3 biological replicates). d 2-day TFP treatment following 6-day ENR + CD differentiation reveals dose-dependent toxicity, with preference to PCs (CD24+ and LYZ+) and PC-like (CD24+ and LYZ+) populations as assessed by flow cytometry (n = 5 biological replicates)

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