Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Table 4 Analysis of the Gckr^P446L project

Founder ID	Allele 1	Allele 2	Allele 3	Copy number	F1 animal ID	PCR and sequencing outcome	Copy number	Allele 1	Allele 2
lss-GckrP446L-11	Legitimate repair	Legitimate repair	Deletion^b	1.47 ± 0.11	11.1a	WT	1.53 ± 0.07	WT	WT?^a
					11.1b	WT	1.03 ± 0.04	WT	Deletion
					11.1c	Legitimate repair and WT	1.88 ± 0.10	WT	Legitimate repair
					11.1d	WT	1.05 ± 0.06	WT	Deletion
					11.1e	WT	1.01 ± 0.05	WT	Deletion
					11.1f	Legitimate repair and WT	1.85 ± 0.06	WT	Legitimate repair
					11.1g	Legitimate repair and WT	1.85 ± 0.12	WT	Legitimate repair
					11.1h	WT	1.01 ± 0.05	WT	Deletion
lss-GckrP446L-19	Legitimate repair	Legitimate repair	Deletion^b	1.44 ± 0.18	19.1a	Legitimate repair and WT	1.90 ± 0.09	WT	Legitimate repair
lss-GckrP446L-19	Legitimate repair	Legitimate repair	Deletion^b	1.44 ± 0.18	19.1b	Legitimate repair and WT	1.81 ± 0.12	WT	Legitimate repair

The table details the results of screening of two positive F₀ animals obtained for the generation of a Gckr^P446L point mutation and the subsequent characterization of the F₁ animals obtained from mating of these F₀ animals to WT mice
^aDeletion affecting the region recognized by the TaqMan™ assay
^bRevealed by copy number

ISSN: 1741-7007