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Table 4 Analysis of the GckrP446L project

From: Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

Founder ID Allele 1 Allele 2 Allele 3 Copy number F1 animal ID PCR and sequencing outcome Copy number Allele 1 Allele 2
lss-GckrP446L-11 Legitimate repair Legitimate repair Deletionb 1.47 ± 0.11 11.1a WT 1.53 ± 0.07 WT WT?a
11.1b WT 1.03 ± 0.04 WT Deletion
11.1c Legitimate repair and WT 1.88 ± 0.10 WT Legitimate repair
11.1d WT 1.05 ± 0.06 WT Deletion
11.1e WT 1.01 ± 0.05 WT Deletion
11.1f Legitimate repair and WT 1.85 ± 0.06 WT Legitimate repair
11.1g Legitimate repair and WT 1.85 ± 0.12 WT Legitimate repair
11.1h WT 1.01 ± 0.05 WT Deletion
lss-GckrP446L-19 Legitimate repair Legitimate repair Deletionb 1.44 ± 0.18 19.1a Legitimate repair and WT 1.90 ± 0.09 WT Legitimate repair
19.1b Legitimate repair and WT 1.81 ± 0.12 WT Legitimate repair
  1. The table details the results of screening of two positive F0 animals obtained for the generation of a GckrP446L point mutation and the subsequent characterization of the F1 animals obtained from mating of these F0 animals to WT mice
  2. aDeletion affecting the region recognized by the TaqMan™ assay
  3. bRevealed by copy number