Unique sperm haplotypes are associated with phenotypically different sperm subpopulations in Astyanax fish

Table 3 Computer simulation of the experimental analysis confirms its efficacy to detect biased haplotypic input

Model	Filter	% of dataset	HET:HOM	HET:HOM	Two-tailed
	Level	Retained	Input as P(HET)	Output	Binomial p =
5 cells	0.2–0.8	14.5	0.3	96:49	5.88E−05
		14.3	0.275	90:53	1.24E−03
		16.5	0.25	82:83	ns
10 cells	0.2–0.8	1.2	0.3	11:1	6.35E−03
		1.8	0.275	13:5	9.63E−02
		1.0	0.25	4:6	ns
10 cells	0.3–0.7	12.6	0.3	92:34	2.36E−07
		14.6	0.275	97:49	8.77E−05
		11.4	0.25	56:58	ns

The results of the simulation for models with 5 and 10 “cells” per “well.” Output bias of HET > HOM was tested using the binomial test. In each of the three simulations, when input was unbiased, no significant bias was exhibited in the output. In contrast, when input was biased (HET > HOM), significant bias of HET > HOM was detected in five of the six tests (p < 0.01)

ISSN: 1741-7007