Fig. 5

Effects of Six1 overexpression on NPB markers and other ectodermal TFs. a–j Expression of PPE (Eya1, Sox3), NC (FoxD3), neural plate (Sox3) markers and early ectodermal TFs in dorsal views of neural plate stage Xenopus embryos after injection of Six1 mRNA. Anterior is to the bottom. Control side is shown on the left and injected side on the right (as indicated by red LacZ immunostaining). Arrowheads indicate expression domains on the control side. Reductions (arrows) and increased or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Insets show alternative phenotypes. k Summary of regulatory interactions. Arrows indicate ability of Six1 to promote expression of TFs. Bars indicate ability of Six1 to repress TFs. Faint colors indicate less frequent phenotypes. See Additional file 1: Table S9 for numbers