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Table 2 Equilibrium binding affinities and kinetic rate constants for binding of Ang2-BD variants to immobilized Tie2 and αvβ3 integrin

From: Targeting the Tie2–αvβ3 integrin axis with bi-specific reagents for the inhibition of angiogenesis

Variant

SPR (immobilized Tie2): steady state

SPR (immobilized αvβ3 integrin): 1:1 Langmuir binding model

KD ± sem, μM

KD ± sem, nM

Kon ± sem, (M−1 s−1) × 104

Koff ± sem, (s−1) × 10−4

Ang2-BDWT

0.66 ± 0.04

*

*

*

Ang2-BDBC5

1.36 ± 0.68

14.9 ± 1.14

2.99 ± 0.01

4.44 ± 0.03

Ang2-BDBC6

1.26 ± 0.18

2.97 ± 0.34

7.94 ± 0.02

2.36 ± 0.01

Ang2-BDBC10

0.95 ± 0.1

4.17 ± 0.54

10.3 ± 0.01

4.29 ± 0.02

  1. Values in the table are means ± SEM. SPR sensorgram curves were fitted to a steady state model (for Tie2 binding) and a 1:1 Langmuir binding model (for αvβ3 integrin binding)
  2. *Binding was not observed for Ang2-BD variants in the 2 μM–125 nM range
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BMC Biology

ISSN: 1741-7007

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