Skip to main content

Table 2 Equilibrium binding affinities and kinetic rate constants for binding of Ang2-BD variants to immobilized Tie2 and αvβ3 integrin

From: Targeting the Tie2–αvβ3 integrin axis with bi-specific reagents for the inhibition of angiogenesis

Variant SPR (immobilized Tie2): steady state SPR (immobilized αvβ3 integrin): 1:1 Langmuir binding model
KD ± sem, μM KD ± sem, nM Kon ± sem, (M−1 s−1) × 104 Koff ± sem, (s−1) × 10−4
Ang2-BDWT 0.66 ± 0.04 * * *
Ang2-BDBC5 1.36 ± 0.68 14.9 ± 1.14 2.99 ± 0.01 4.44 ± 0.03
Ang2-BDBC6 1.26 ± 0.18 2.97 ± 0.34 7.94 ± 0.02 2.36 ± 0.01
Ang2-BDBC10 0.95 ± 0.1 4.17 ± 0.54 10.3 ± 0.01 4.29 ± 0.02
  1. Values in the table are means ± SEM. SPR sensorgram curves were fitted to a steady state model (for Tie2 binding) and a 1:1 Langmuir binding model (for αvβ3 integrin binding)
  2. *Binding was not observed for Ang2-BD variants in the 2 μM–125 nM range