Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns

Table 1 Diffusion coefficients of 25 selected proteins of B. subtilis grown at 23 °C, 37 °C, or 43 °C

Protein	kDa	TMs	23 °C		37 °C		43 °C
Protein	kDa	TMs	μm²/s	% tracks	μm²/s	% tracks	μm²/s	% tracks
DivIVA	19.2	0/	0.00	53	0.01	39	0.00	41
DivIVA		Associated*	0.37	47	0.60	61	0.63	59
EfeU	52.24	6	0.00	55	0.01	52	0.01	53
EfeU			0.34	45	0.41	48	0.42	47
FtsH	70.76	2	0.00	59	0.00	47	0.00	46
FtsH			0.55	41	0.82	53	0.85	54
GltP	44.45	12	0.00	57	0.00	70	0.00	74
GltP			0.42	43	0.41	30	0.39	26
GuaB	52.82	0/	0.10	26	0.18	23	0.20	18
GuaB		Cytoplasmic	0.69	74	0.89	77	0.96	82
LmrB	51.54	14	0.00	55	0.00	57	0.00	60
LmrB			0.28	45	0.27	43	0.30	40
MinJ	43.51	7	0.00	60	0.00	45	0.00	46
MinJ			0.27	40	0.50	55	0.53	54
OpuAB	30.1	7	0.01	64	0.02	60	0.04	63
OpuAB			0.44	36	0.42	40	0.46	37
YbaF	29.54	6	0.00	58	0.02	56	0.01	54
YbaF			0.31	42	0.44	44	0.49	46
YbfF	35.16	4	0.00	50	0.00	39	0.00	46
YbfF			0.37	50	0.65	61	0.68	54
YbxA	31.31	0/	0.00	58	0.04	55	0.04	59
YbxA		Associated	0.34	42	0.49	45	0.62	41
YceF	29.02	6	0.00	55	0.00	57	0.00	54
YceF			0.50	45	0.59	43	0.63	46
YceJ	41.8	12	0.00	62	0.00	56	0.00	58
YceJ			0.18	38	0.26	44	0.22	42
Ycgq	33.06	4	0.00	58	0.01	55	0.01	62
Ycgq			0.49	42	0.55	45	0.60	38
YcgR	31.78	8	0.00	54	0.00	64	0.01	59
YcgR			0.33	46	0.39	36	0.42	41
YciC	45.15	0/	0.00	53	0.01	44	0.01	39
YciC		Associated	0.60	47	0.84	56	0.79	61
YdbL	12.79	4	0.01	63	0.02	51	0.02	45
YdbL			0.38	37	0.60	49	0.58	55
YddI	18.86	1	0.01	58	0.02	51	0.02	54
YddI			0.50	42	0.80	49	0.71	46
YknZ	41.96	4	0.00	53	0.00	45	0.00	50
YknZ			0.22	47	0.57	55	0.68	50
YvcK	34.53	0/	0.02	48	0.05	42	0.06	43
YvcK		Cytoplasmic	0.59	52	0.97	58	0.90	57
YxcA	9.7	2	0.00	50	0.00	53	0.00	54
YxcA			0.23	50	0.58	47	0.60	46
YxeN	24.86	6	0.00	47	0.01	52	0.01	57
YxeN			0.28	53	0.39	48	0.47	43
YycG	69.86	2	0.01	47	0.00	42	0.00	43
YycG			0.55	53	0.66	58	0.79	57
ZnuA	35.51	0/	0.01	63	0.01	57	0.02	58
ZnuA		Lipid anchor	0.50	37	0.53	43	0.55	42
ZnuB	30.28	7	0.00	79	0.01	71	0.01	74
ZnuB			0.42	21	0.49	29	0.47	26

Percentages of tracks show there are two different diffusing populations for each protein. Localization error in our system corresponds to a movement of 0.006 μm²/s, determined movement close to this number is stated as “0.00”. Standard deviations for all diffusions are shown in Additional file 6
*Membrane associated

ISSN: 1741-7007