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Table 1 Diffusion coefficients of 25 selected proteins of B. subtilis grown at 23 °C, 37 °C, or 43 °C

From: Microdomain formation is a general property of bacterial membrane proteins and induces heterogeneity of diffusion patterns

Protein kDa TMs 23 °C   37 °C   43 °C  
μm2/s % tracks μm2/s % tracks μm2/s % tracks
DivIVA 19.2 0/ 0.00 53 0.01 39 0.00 41
  Associated* 0.37 47 0.60 61 0.63 59
EfeU 52.24 6 0.00 55 0.01 52 0.01 53
   0.34 45 0.41 48 0.42 47
FtsH 70.76 2 0.00 59 0.00 47 0.00 46
   0.55 41 0.82 53 0.85 54
GltP 44.45 12 0.00 57 0.00 70 0.00 74
   0.42 43 0.41 30 0.39 26
GuaB 52.82 0/ 0.10 26 0.18 23 0.20 18
  Cytoplasmic 0.69 74 0.89 77 0.96 82
LmrB 51.54 14 0.00 55 0.00 57 0.00 60
   0.28 45 0.27 43 0.30 40
MinJ 43.51 7 0.00 60 0.00 45 0.00 46
   0.27 40 0.50 55 0.53 54
OpuAB 30.1 7 0.01 64 0.02 60 0.04 63
   0.44 36 0.42 40 0.46 37
YbaF 29.54 6 0.00 58 0.02 56 0.01 54
   0.31 42 0.44 44 0.49 46
YbfF 35.16 4 0.00 50 0.00 39 0.00 46
   0.37 50 0.65 61 0.68 54
YbxA 31.31 0/ 0.00 58 0.04 55 0.04 59
  Associated 0.34 42 0.49 45 0.62 41
YceF 29.02 6 0.00 55 0.00 57 0.00 54
   0.50 45 0.59 43 0.63 46
YceJ 41.8 12 0.00 62 0.00 56 0.00 58
   0.18 38 0.26 44 0.22 42
Ycgq 33.06 4 0.00 58 0.01 55 0.01 62
   0.49 42 0.55 45 0.60 38
YcgR 31.78 8 0.00 54 0.00 64 0.01 59
   0.33 46 0.39 36 0.42 41
YciC 45.15 0/ 0.00 53 0.01 44 0.01 39
  Associated 0.60 47 0.84 56 0.79 61
YdbL 12.79 4 0.01 63 0.02 51 0.02 45
   0.38 37 0.60 49 0.58 55
YddI 18.86 1 0.01 58 0.02 51 0.02 54
   0.50 42 0.80 49 0.71 46
YknZ 41.96 4 0.00 53 0.00 45 0.00 50
   0.22 47 0.57 55 0.68 50
YvcK 34.53 0/ 0.02 48 0.05 42 0.06 43
  Cytoplasmic 0.59 52 0.97 58 0.90 57
YxcA 9.7 2 0.00 50 0.00 53 0.00 54
   0.23 50 0.58 47 0.60 46
YxeN 24.86 6 0.00 47 0.01 52 0.01 57
   0.28 53 0.39 48 0.47 43
YycG 69.86 2 0.01 47 0.00 42 0.00 43
   0.55 53 0.66 58 0.79 57
ZnuA 35.51 0/ 0.01 63 0.01 57 0.02 58
  Lipid anchor 0.50 37 0.53 43 0.55 42
ZnuB 30.28 7 0.00 79 0.01 71 0.01 74
   0.42 21 0.49 29 0.47 26
  1. Percentages of tracks show there are two different diffusing populations for each protein. Localization error in our system corresponds to a movement of 0.006 μm2/s, determined movement close to this number is stated as “0.00”. Standard deviations for all diffusions are shown in Additional file 6
  2. *Membrane associated