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Fig. 7 | BMC Biology

Fig. 7

From: Parkinson’s disease-linked Parkin mutations impair glutamatergic signaling in hippocampal neurons

Fig. 7

Parkin mutations reduce Homer1 binding and synaptic retention. a Representative Parkin and Homer1 immunoblots for Homer1 IP from HEK293T cell lysates expressing GFP-Homer1 and GFP-Parkin WT/T240M/R275W/R334C/G430D. b Quantification of Homer1/Parkin interaction by measurement of co-immunoprecipitated GFP-Parkin WT/T240M/R275W/R334C/G430D, normalized to immunoprecipitated GFP-Homer1 and reported as a fraction of GFP-Parkin WT condition (n = 3 experiments, one-way ANOVA, *P < 0.05, **P < 0.01, error bars represent SEM). c Representative images of Homer1 (red) and VAMP2 (blue) staining in 14–16 DIV hippocampal neurons expressing GFP, shParkin, or shParkin-WT/-T240M/-R275W/-R334C/-G430D. Arrows indicate representative synaptic Homer1 puncta (based on co-localization with VAMP2) for each condition. Scale bar, 10 μm. d Quantification of synaptic Homer1 intensity at VAMP2-immunopositive puncta along dendrites, expressed as a fraction of shParkin-WT control condition (n ≥ 50 fields of view per condition with > 50 co-localized Homer1 puncta per field, results confirmed in 3 independent experiments; ***P < 0.001, one-way ANOVA, error bars represent SEM)

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