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Fig. 4 | BMC Biology

Fig. 4

From: Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance

Fig. 4

Global transcription is altered in offspring of Eedhypo/hypo males. a Heatmap of 1986 differentially expressed transcripts (P < 0.01, ≥ 1.5-fold change; Benjamini-Hochberg false detection correction) detected using RNA-seq. Each column represents an RNA sample from an individual heterozygous embryo sired by either a homozygous (Eedhypo/hypo) or heterozygous (Eedhypo/wt) male. Three different Eed littermate pairs (i.e. Eedhypo/hypo and Eedhypo/wt brothers) were used to generate progeny for each genotype. b Heat map of 112 differently expressed genes detected using both Agilent 8x60K arrays and RNA-seq (P < 0.01; ≥ 2-fold change; Benjamini-Hochberg false detection correction for arrays and RNAseq). Each column (1–4) represents an RNA sample from an individual heterozygous embryo sired by either an Eedhypo/hypo or an Eedhypo/wt male. c Pie chart illustrating functional classification of differentially regulated genes detected using micro-array and RNA-seq. Processed retrotransposed pseudogenes (P < 4 × 10− 7, Fisher exact test) and lincRNAs (P < 0.05, Fisher exact test) were significantly enriched in genes upregulated in Eedhypo/wt progeny of Eedhypo/hypo compared to Eedhypo/wt males

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