Fig. 2
From: VOPP1 promotes breast tumorigenesis by interacting with the tumor suppressor WWOX
Subcellular localization of WWOX, VOPP1, and WWOX/VOPP1 complex in Hela cells. a Cells were transfected with the WWOX expression vector. The overlay of immunostaining with anti-WWOX and anti-GM130 antibodies indicates a localization of WWOX protein in the Golgi apparatus. b Cells were transfected with VOPP1. VOPP1 labeling coincides with LAMP2 patterns but not with GM130 or EE1A distribution suggesting lysosomal localization of VOPP1 protein. c Hela cells were transfected with WWOX and VOPP1 (left panels), or WWOX and VOPP1Y165A (right panels) expression vectors as indicated. After immunostaining with anti-VOPP1, anti-WWOX, and anti-GM130, cells were counterstained with DAPI. WWOX and VOPP1 colocalization was observed (yellow) in cells expressing VOPP1, but not VOPP1Y165A. All experiments were performed using 3D deconvolution microscopy and digital overlay of images (original magnification, × 100)