Fig. 2

Distinct cnidocyte populations. Panels a and c highlight the FACS-assisted sorting strategies employed in this study. The cells are first sorted into Hoechst-negative and Hoechst-positive populations, followed by the separation of the latter into memOrange2-positive, memOrange2-super-positive, and memOrange2-negative populations. Panels b and d depict images of sorted positive and super-positive cells, as observed in differential interference contrast (DIC), mOrange, and DAPI filters, respectively, under a fluorescent microscope. A merger of these three images is also shown. Scale bar is 20 μm in b and d