Fig. 6

β1tubulin-containing MTs regulate PolyE-MT fasciculation and coiling in PPLL. a Total protein extracts of mouse platelets and Luc-, β1tubulin-, and TTLL5-depleted polyploid D723H cells analyzed for indicated antibodies. β1tubulin and TTLL5 are partially depleted following siRNA treatment, and depletions do not affect Ac-MTs levels. β1tubulin depletion does not affect polyE-MTs while TTLL5 does. b, c Luc-, β1tubulin, and TTLL5-depleted polyploid D723H cells stained for total tubulin, βtubulin, and PolyE-MTs after 16 h spreading on fibrinogen. b Quantification of number of elongations per cell and several shape descriptors using FIJI software (n = 3, at least 150 cells were analyzed per condition). Error bars are SEM (p values < 0.0001, two-tailed t test for each test). c Representative images of luc-, β1tubulin-, TTLL5, and low TTLL5-depleted cells stained for tubulin β1tubulin and PolyE-MTs (bars, 50 μm and 10 μm for high magnification insets)