Skip to main content
Fig. 5 | BMC Biology

Fig. 5

From: Migration through a small pore disrupts inactive chromatin organization in neutrophil-like cells

Fig. 5

An example region of chromosome 17(q24.1-q25.1) showing genome organization features following migration with or without constriction. a Significant contacts (FDR < 0.05, 100 kb resolution) in unmigrated cells, and cells migrated with or without constriction. Line thickness indicates significance. b Hi-C heatmap PC1 values, indicating compartments A (positive, black) and B (negative, grey) in the three conditions. c Correlation values show how similar the contact patterns are between two conditions. Comparisons between all three conditions are shown here, with a dotted line at R = 0.6 indicating the cutoff used to call compartment switching. d Domain (TAD) locations for all three conditions are shown here. The boundaries of TADs that are not conserved between migration with and without constriction are indicated with dashed grey lines. e The positions of expressed genes, and log mean gene expression of the two migration conditions. One gene in this region was differentially expressed (red) between migration with and without constriction; however, it was in a region with stable compartments, TADs, and contacts. f Hi-C heatmaps of the ICE normalized contact frequency at 128 kb resolution, visualized in HiGlass [100]. Lighter colour indicates higher contact frequency between regions in i unmigrated cells, ii cells migrated without constriction, and iii cells migrated with constriction

Back to article page