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Fig. 6 | BMC Biology

Fig. 6

From: Mitochondrial unfolded protein response transcription factor ATFS-1 promotes longevity in a long-lived mitochondrial mutant through activation of stress response pathways

Fig. 6

ATFS-1 mediates specific transcriptional changes in nuo-6 worms. Quantitative real-time RT-PCR was used to compare the expression of selected stress response and metabolism genes between nuo-6 and nuo-6;atfs-1 worms. a nuo-6 worms showed decreased expression of hsp-4, which is a marker of the endoplasmic reticulum unfolded protein response. b hsp-6 expression was increased in nuo-6 worms and this increase was completely prevented by loss of atfs-1. c Deletion of atfs-1 resulted in increased expression of hsp-16.2, which is a marker of the cytosolic unfolded protein response. d nuo-6 worms exhibited increased expression of the SKN-1/NRF2 target gene gst-4, which was prevented by atfs-1 deletion. e Expression of the HIF-1 target gene nhr-57 is increased in nuo-6 worms and restored towards wild-type by the atfs-1 deletion. The DAF-16/FOXO target genes mtl-1 (f), sodh-1 (g), and sod-3 (h), are upregulated in nuo-6 worms and require atfs-1 for this upregulation. Similarly, fmo-2 (i) and cdr-2 (j), which are involved in xenobiotic detoxification, exhibit an ATFS-1-dependent increase in expression in nuo-6 mutants. kn Selected genes involved in metabolism (ldh-1, aldo-1, gpd-2, acs-2) show an ATFS-1-dependent increase in expression in nuo-6 worms. Error bars indicate SEM. *p < 0.05,**p < 0.01,***p < 0.001. See Additional file 5: Table S5 for details on N and replicates

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