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Fig. 7 | BMC Biology

Fig. 7

From: A role for actomyosin contractility in Notch signaling

Fig. 7

Basal protrusions and mechanical force. PIV analysis was performed after addition of either (a, b) control (dH2O) or (c, d) 0.1 mM Y27632. (b, d) Quantification of mean vector magnitude for each frame pair (1 frame captured every 10 s) after control or drug addition. Comparison of best-fit slope relative to control, ***p < 0.0001. e Basal view of live nota expressing UAS-LifeActRuby under pnr-GAL4 in all signaling cells during a laser ablation protocol. Dashed yellow line indicates location and length of cut target. Time, in ms, at lower right. Apical view post-cut indicates that the apical regions of cells were not affected in the area in which the basal surface was targeted (yellow box). Scale bars: basal view, 10 μm; apical view, 25 μm. f Quantification of mean initial recoil post-laser ablation of the basal protrusion network for control (UAS-LifeActRuby; n = 35 cuts; 10 pupae) or spaghetti squashAA (n = 22 cuts; 8 pupae) expressing nota. ***p ≤ 0.001 by unpaired t test. See also Additional file 7: Movie S3

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