Fig. 3
From: A deletion in the RD105 region confers resistance to multiple drugs in Mycobacterium tuberculosis
Rv0071/74 binds with and cleaves peptidoglycan (PGN). a Purified protein Rv0071/74, Rv0074 and Rv0071/74-AS1 mutant in SDS-PAGE gel stained with Coomassie blue. M: protein ladder. b, c OD450 analysis (Additional file 4: Table S5) (b) or liquid chromatography analysis (c) of PGN-S. aureus (S) or PGN-M. smegmatis (M) incubated with purified Rv0071/74 protein. Data shown are representative of three independent experiments. Error bars, means ± SD. F: full-length; T: truncated. d Processed kinetic interaction of WxL domain with PGN including baseline (phase I), loading the WxL peptide (phase II), removing unbound peptide (phase III), association of peptide and PGN (phase IV), and dissociation of peptide and PGN (phase V)