Fig. 8

Oocyte-specific deletion of Gsk-3β disrupted early folliculogenesis in mice. a Gsk-3β was efficiently and specifically deleted in the oocytes in cKO mouse ovary. Immunofluorescence staining for GSK-3β (green) and DDX4 (red) on 1 dpp ovary. The nucleus was stained by Hoechst (blue). b Control and cKO ovaries at the indicated developmental stages. Oocytes were stained with DDX4 (green). The nucleus was stained by Hoechst (blue). c Statistical analysis showed that the total number of primordial follicle decreased significantly in 7 dpp cKO ovaries (Additional file 8: Individual data values). d Apoptotic cells increased in 1 dpp cKO ovaries compared with the control ovaries. TUNEL signals (green) marked apoptotic cells. The nucleus was stained by Hoechst (blue). e DSBs persisted in the oocytes of 1 dpp cKO ovaries. The sections were stained with γ-H2AX (green) and DDX4 (red). The nucleus was stained by Hoechst (blue). f Ectopic RAD51 expression in the oocytes of 1 dpp cKO ovaries. The sections were stained with RAD51 (green) and DDX4 (red). The nucleus was stained by Hoechst (blue). g, h β-catenin accumulated in the cytoplasm and translocated into the nucleus of the oocytes in cKO mice. g The sections were stained with β-catenin (green) and DDX4 (red). The nucleus was stained by Hoechst (blue). Arrowheads indicated oocytes showing nuclear β-catenin accumulation. h The statistic analysis demonstrated that the percentage of oocytes showing β-catenin accumulation per section increased significantly in cKO mice (Additional file 8: Individual data values). The data are presented as mean ± s.d. The asterisk (*) denotes a statistically significant difference between the control and treatment groups. *P < 0.05, **P < 0.01, and ***P < 0.001 (t test). Scale bars, 200 μm