Fig. 5

Knockdown of ARGs by RNAi affected social effects on daytime sleep. Knock-down of ARGs in dopaminergic neurons was achieved by driving RNAi transgenes with TH-GAL4; controls carried empty vectors without RNAi hairpin and TH-GAL4. a Example graph of sleep per 30 min over 24 h. Control single housed (SH) flies sleep less than group housed (GH) flies during the day (shaded gray area). Expressing RNAi for ARG-TF cabut in dopaminergic neurons significantly reduced this difference. b Daytime sleep was measured, and ΔSleep was compared between experimental males carrying the RNAi transgene and controls. ΔSleep is defined as minutes of daytime sleep for GH flies minus the same measure for SH flies (as described by Ganguly et al. [11]). c ΔSleep for controls and RNAi knockdowns. Error bars are mean ± SEM. In every case, RNAi knockdown significantly reduced the social effect on ΔSleep. Two different RNAi lines were tested for CrebA, each showing significant reductions