Fig. 2

Experimental expression distributions of the self-repressing gene circuit (MG::PR-8 T) at different aTc concentrations observed under a microscope. a In M9 media with the inducer concentrations ranging from 300 to 1500 ng/mL of aTc, the resulting steady state fluorescence distributions show that the ratio of the populations of the bimodal fluorescence distributions depend on the aTc concentration. Seven color histograms represent different inducer concentrations. b–e Four representative fluorescence images at different concentrations of aTc (300, 700, 1000, and 1500 ng/mL) are selected