Fig. 4

The mean fluorescence intensity distribution of the dynamical trajectories for MG::PR-8 T. Single-cell mean fluorescence intensities were captured every 5 min. 28 micro-colonies were tracked by time-lapse microscopy. a Three representative single cell fluorescence trajectories induced by 1500 ng/mL aTc. Points represent experimental fluorescence values. Red vertical dashed lines demarcate cell divisions. b The bright field and fluorescent field images of the corresponding measurements in the time-lapse experiment. The cells corresponding to the fluorescence trajectory in Fig. 4a are marked with red circles. The average of bacteria mean fluorescence intensity is 556, and the average cell cycle time is 46 min in this micro-colony. c The histogram gives the intensity distribution of the 163 single-cell fluorescence trajectories induced at 1500 ng/mL aTc collected from the time-lapse experiments. The red solid curve is the fitted intensity distribution from HMM