Fig. 3
From: mTORC1/AMPK responses define a core gene set for developmental cell fate switching
Identification of media for rapid growth but permissive to rapamycin-induced development. a Log-phase cells in full growth media were adhered to cell well plates in Glc[-] media at 100% or 50% strength; after 2 h, fresh media were replaced with and without rapamycin. After 10 h, were analyzed by immunoblotting. Immunoblots are representative of three independent experiments. b Log-phase cells in full growth media were adhered to cell well plates in full media. After 2 h, media were replaced with fresh full media, DB, Glc[-] media at 100%, or Glc[-] media at 50%, with and without rapamycin. After 10 h, were visualized for developmental aggregation. c Log-phase cells in full growth media were adhered to cell well plates in Glc[-]media at 100% or 50% strength, with varying concentrations of glucose. After 2 h, media were replaced with and without rapamycin. After 10 h, samples were analyzed by immunoblotting. Immunoblots are representative of three independent experiments. d Log-phase cells in full growth media were adhered to cell well plates in full media. After 2 h, media were replaced with fresh full media, DB, Glc[-] media at 50%, with glucose as indicated, and with or without rapamycin as indicated. Developmental aggregation was visualized. GDT media is defined as 50% Glc[-] Media + 27 mM glucose