Fig. 2
From: FoxH1 represses miR-430 during early embryonic development of zebrafish via non-canonical regulation
FoxH1 regulates miR-430 expression in a negative manner. a Genomic organization of the miR-430-containing region on chromosome 4. FoxH1 peaks localize to the miR-430a, b, and c repeat cluster. Enlargements indicate positions of transcripts for miR-430a (red), miR-430b (green) and miR-430c (blue) which were directly extracted from the UCSC browser [45]. b Temporal changes of mature miR-430 levels in MZsur embryos between 4hpf and 9hpf as measured by RT-qPCR relative to wild-type embryos. Starting at 5hpf, levels of mature miR-430 are increased in the mutant. c The upregulation of pri-miR-430 forms in MZsur mutants can be partly rescued by injection of foxH1WT mRNA. Addition of FoxH1 in wild types does not influence the expression level. d All three pri-miR-430 isoforms are upregulated in MZsur mutants at the indicated time points. Loss of Nodal signaling in the oep mutant does not significantly change expression levels. All data normalized to respective wild type control. e Control RT-qPCR shows for MZsur and MZoep mutants a highly significant downregulation of gsc expression as formerly shown [11, 46]. All qPCR data were calculated from biological triplicates except for b (2 biological replicates). Error bars indicate standard error (SEM). Bio-Rad CFX Manager 3.1 software was used to calculate relative normalized expression, standard error, and significance (n. s. p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001). For individual values, see also Additional file 5: Individual qPCR values