Fig. 2.

Wound healing by filopodia-dependent cell crawling. a Schematic representation of the puncture assay. b–e DIC images of the main phases of wound closure (see Additional file 1 for the time-lapse video corresponding to these images). b Cells from the mesoglea (yellow arrow caps) migrate upwards and adhere to the edges of the wound. c Marginal cells of the wound gap extend filopodia to the adjacent cells pulling the edges of the wound together. The inset shows a closer look to the cells at the edge of the wound, and yellow arrow caps point to the filopodia. d When the diameter of the gap is considerably reduced, cells of the wound edge extend filopodia towards the opposite edges of the gap. The inset shows a cell extending multiple filopodia. e Network of filopodia connecting all the edges of the wound. Timescale is in hours. Scale bars = 100 μm