Fig. 7.

eRNAi phenotypes in Nv-bark beetle dsRNA-injected embryos. A1–A3’ Pre-blastoderm stage. B1–B3’ Early blastoderm stage. C1–C3’ Mid-blastoderm stage. D1–D3’ Mid-blastoderm stage. In each quadrant, the topmost image (A1, B1, C1, D1) shows a single-channel image of fluorescent in situ hybridization signal using a probe against Nv-nanos. The next image down contains single-channel images of DAPI staining (A2, B2, C2, D2). The merges of two single channels are in the bottom image of each quadrant (A3, B3, C3, D3). Enlarged views of the posterior pole (marked by white boxes in the whole embryo panels) appear to the right of each image (A1’–D3’). The white arrowheads indicate areas of injection damage in the embryos. The green arrow head indicates the free germ plasm in the embryo, and yellow arrowheads indicate germ plasm associated with a nucleus. All embryos are positioned with the posterior side to the right and dorsal side on the top. Scale bar indicates 50 μm