Fig. 5
FLAG-p150 co-purifies with density aggregation activity. a/b Conditioned media from N-FLAGOE cells was fractionated on mono Q and eluate fractions assayed by immunoblot for FLAG protein (a) and for density-dependent aggregation activity (b) using WT cells at 20 × 103 cells/cm2. c Upper panels—Mono Q fractionated media from DPFOE or N-FLAGOE cells were assayed for density-dependent aggregation activity using WT cells at 20 × 103 cells/cm2. Lower panels—media were affinity purified with α-FLAG and re-assayed at varying dilutions for density-dependent aggregation activity using WT cells at 20 × 103 cells/cm2