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Fig. 5 | BMC Biology

Fig. 5

From: DPF is a cell-density sensing factor, with cell-autonomous and non-autonomous functions during Dictyostelium growth and development

Fig. 5

FLAG-p150 co-purifies with density aggregation activity. a/b Conditioned media from N-FLAGOE cells was fractionated on mono Q and eluate fractions assayed by immunoblot for FLAG protein (a) and for density-dependent aggregation activity (b) using WT cells at 20 × 103 cells/cm2. c Upper panels—Mono Q fractionated media from DPFOE or N-FLAGOE cells were assayed for density-dependent aggregation activity using WT cells at 20 × 103 cells/cm2. Lower panels—media were affinity purified with α-FLAG and re-assayed at varying dilutions for density-dependent aggregation activity using WT cells at 20 × 103 cells/cm2

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