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Fig. 2 | BMC Biology

Fig. 2

From: Functional architecture underlying binocular coordination of eye position and velocity in the larval zebrafish hindbrain

Fig. 2

Experimental strategy to assess binocular coordination. a: Example stimulus protocol (from recording shown in a’). Lines indicate direction in which the stimulus is moving. Dashed lines separate stimulus phases. a′ Example eye traces (right eye (RE): magenta, left eye (LE): cyan) and corresponding neuronal calcium responses (black, ΔF/F) with monocular coding. The respective highest scoring regressor [monocular right eye, rightward eye position (r3); monocular left eye, rightward eye position (r7)] is shown in blue. Grey line shows right eye position from which r3 was derived. b Example eye traces with derived binocular regressors (top 3 plots) and calcium responses of binocular neurons in the same recording (lower 2 plots). Left and right eye traces with capped counter-clockwise eye velocity (grey, upper two plots) and averaged eye position (grey, third plot) of which regressors 18 (binocular always leftward position, blue trace in third and fourth plot) and r24 (binocular always leftward velocity, blue trace in fifth plot) were derived. Black lines show ΔF/F (DFF) calcium signals for a binocular always (BA) position (P) and a BA velocity (V) neuron with the corresponding highest scoring regressor in blue. b′ Example binocular preferred (BP) position neuron with respective eye trace; note the binocular event during the LE stimulation (red arrow). The blue trace shows the respective regressor (binocular preferred, rightward position, r1), the red trace the corresponding velocity regressor (binocular preferred, rightward velocity, r9). c Overview of kinematic eye parameters addressed in this study to classify response types and regressor overview. Left, depiction of kinematic parameters. Middle, response types. Each set of three squares corresponds to one type of regressor, see colour legend. Right, full list of regressors in grey. Italic numbers correspond to averaged regressors; BE, both eyes; MLEX, monocular left eye exclusive; MREX, monocular right eye exclusive

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