Fig. 2
From: The role of T cell trafficking in CTLA-4 blockade-induced gut immunopathology
Increase in gut-homing CD4+ T cell numbers induced by anti-CTLA-4 therapy is independent of CD103. a Knocking out CD103 decreased β7 expression in blood CD4+ and CD8+ T cells. Flow cytometric analysis of activated gut-homing T cells in the blood from WT and CD103 KO mice. Representative flow plots depicting activated gut-homing T cells (β7+CD44+) (left) and quantification of activated gut-homing T cells (right). The upper profiles were gated on live CD4+ T cells, and the lower profiles were gated on live CD8+ T cells. b The increase in gut-homing CD4+ T cell numbers by anti-CTLA-4 therapy was independent of CD103. Flow cytometric analysis of activated gut-homing CD4+ T cells in the blood 10 days after isotype antibody (Ctrl) (upper profiles) or anti-CTLA-4 mAb (lower profiles) treatment from WT and CD103 KO mice. The left profiles are representative flow plots depicting activated gut-homing T cells (β7+CD44+), and the right bar graph shows the fold change in activated gut-homing T cells (β7+CD44+) between the anti-CTLA-4 group and the IgG group. There were 5 mice in each group, data represent mean ± SEM, ***P < 0.001