Fig. 1
Suppression of TBPH in muscle affects locomotion and life span. a Number of peristaltic waves of Ctrl (+/+;driverGAL4/UAS-GFP-IR), TBi (+/+;driverGAL4/UAS-TBPH-IR), Ctrl-Δtb/+ (tbphΔ23/ +;driverGAL4/UAS-GFP-IR), and TBi-Δtb/+ (tbphΔ23/+;driverGAL4/UAS-TBPH-IR) using MHC-GAL4 (left panel) and Mef2-GAL4 (right panel). n = 20. b Climbing assay of Ctrl-Δtb/+ and TBi-Δtb/+ using MHC-GAL4 (left panel) and Mef2-GAL4 (right panel) at day 7. n = 200. c Life span analysis of TBi-Δtb/+ compared to Ctrl-Δtb/+ using Mef2-GAL4. n = 200. d Confocal images of the third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) and anti-Dlg (in red) in MHC-Ctrl (tbphΔ23/+;MHC-GAL4/UAS-GFP-IR) and MHC-TBi (tbphΔ23/+;MHC-GAL4/UAS-TBPH-IR). e Quantification of Dlg intensity normalized on Ctrl. n > 200 boutons. f Confocal images of the third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) and anti-GluRIIA (in red) in MHC-Ctrl and MHC-TBi. g Quantification of GluRIIA intensity normalized on Ctrl. n > 200 boutons. h Confocal images of the third instar NMJ terminals in muscle 6/7, second segment stained with anti-HRP (in green) in MHC-Ctrl and MHC-TBi. The details of the bouton shape are shown. i Quantification of the bouton shape. n = 200. j Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) in MHC-Ctrl and MHC-TBi. k Quantification of bouton number. n = 15. l Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) and anti-Futsch (in red) in MHC-Ctrl and MHC-TBi. m Quantification of Futsch intensity normalized on Ctrl. n > 200 boutons. ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001 calculated by one-way ANOVA (for more than two groups) and t test (for two groups) and log rank test (for survival analysis) error bars SEM. Scale bar 10 μm (panel j) and 5 μm (in panels d, f, h, and l)