Fig. 2
The muscular function of TBPH is sufficient to promote neuromuscular synaptic growth. a Number of peristaltic waves of Ctrl (w1118), Δtb-GFP (tbphΔ23/tbphΔ23;driver-GAL4/UAS-GFP), Δtb-TBPH (tbphΔ23,UAS-TBPH/tbphΔ23;driver-GAL4/+), Δtb-hTDP-43 (tbphΔ23/tbphΔ23;driver-GAL4/UAS-TDP-43), and Δtb-TBPHF/L (tbphΔ23/tbphΔ23;driver-GAL4/UAS-TBPHF/L) using Mhc-GAL4 (left panel) and Mef2-GAL4 (right panel). n = 20. b Climbing assay of Ctrl, Δtb-GFP, and Δtb-TBPH using Mef2-GAL4 at day 2. n = 200. c Walking assay analysis of Δtb-GFP and Δtb-TBPH using Mef2-GAL4 at day 2. n = 100. d Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) in Ctrl, Δtb-GFP, and Δtb-TBPH. e Quantification of branch number. n = 15. f Quantification of the bouton shape. n = 200. g Evoked neurotransmitter release. Representative EJPs evoked by segmental nerve stimulation of Ctrl, Δtb-GFP, and Δtb-TBPH in muscle fiber 6/7 of A3 in third instar larvae. For each fiber, 15 EPPs following 0.5-Hz stimulation were considered. h Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) and anti-Dlg (in red) in Ctrl, Δtb-GFP, and Δtb-TBPH. i Quantification of Dlg intensity normalized on Ctrl. n > 200 boutons. j Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) and anti-GluRIIA (in red) in Ctrl, Δtb-GFP, and Δtb-TBPH. k Quantification of GluRIIA intensity normalized on Ctrl. n > 200 boutons. *p < 0.05, **p < 0.01, and ***p < 0.001 calculated by one-way ANOVA, error bars SEM. Scale bar 10 μm (in d) and 5 μm (in h and j)