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Fig. 2 | BMC Biology

Fig. 2

From: Persistent DNA damage triggers activation of the integrated stress response to promote cell survival under nutrient restriction

Fig. 2

Selective survival advantage of XRCC1 KD cells under nutrient restriction depends on ATF4. Phase-contrast images of Tig-1 cells treated with siControl (a), siXRCC1 (b), siATF4 (c), or siXRCC1 and siATF4 (d) and grown in a medium containing 1% FCS. Images are from one representative experiment, with four different fields randomly chosen per condition. Scale bar = 400 μm. e Quantification of relative cell area as shown in ad, normalised to siControl grown at 1% FCS. n = 4. Only statistically significant differences are indicated using asterisks. f Relative mRNA levels of XRCC1, ATF4, PSAT1, ACTA2, and PALLD after siXRCC1 in cells cultured at 15, 5, and 1% FCS, respectively, normalised to expression in the respective control cells. n = 4

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