Fig. 6

S10 relocation effects are reduced in hyperosmotic conditions. a Growth rates of the parental and the indicated movant strains in LB with increasing NaCl concentrations were quantified by averaging the obtained μ in 6 independent experiments with at least 3 biological replicates. The growth of each movant was normalized to the μ of the parental strain, and the percentage of the variation (μ %) ± SEM with respect to parental strains is shown as a function of NaCl concentration of growth medium. b Changes in growth of the movant strains with respect to parental strain are shown as a function of sucrose concentration. Data was treated as in a, but results correspond to 4 independent experiments with at least 3 biological replicates. c MFA profiles are plotted as in Fig. 3b. Results for the parental (purple), the S10Tnp-1120 (red), and the S10TnpC2+479 (blue) strains in LB in the presence of 5 g/L (LB, left panel) or 20 g/L (LB + NaCl, center panel) are shown. The arrow highlights the S10 position in the abscissa, reflecting S10 dosage alterations. The right panel corresponds to MFA of the parental strain when NaCl concentration is 5 or 20 g/L (LB or LB + NaCl). d Replication dynamics in the presence of 5 or 20 g/L of NaCl assessed by calculating the slope for each replichore for 2 independent MFA experiments. Dots indicate mean ± SD. Statistical significance was analyzed by one-way ANOVA two-tailed test and Tukey’s test for multiple comparisons. Significance is indicated as follows: n.s., non-significant; *p < 0.05 and **p < 0.01