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Fig. 7 | BMC Biology

Fig. 7

From: Mapping DNA interaction landscapes in psoriasis susceptibility loci highlights KLF4 as a target gene in 9q31

Fig. 7

qPCR results for genes within the 9q31.2 locus in HaCaT cells expressing dCas9-P300. HaCaT cells expressing dCas9-P300 were transduced with pools of plasmids containing sgRNA targeting psoriasis SNPs (pools 1–4), a scrambled sgRNA (Scr) or the same plasmid without a specific guide cloned in (plasmid only (PO)). TaqMan qPCR results are shown for RAD23B, KLF4, IKBKAP, FAM206A and CTNNAL1. Housekeeping genes used were TBP and YWHAZ. For statistical analysis, a Kruskal-Wallis test was performed comparing the fold changes between cells with the scrambled guide or the sgRNA pools, using Dunn’s multiple comparisons test to identify significant differences. Asterisks denote P < 0.05. Graphs show the mean fold change in comparison with the scrambled guide, ± SD of biological triplicate cell lines

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BMC Biology

ISSN: 1741-7007

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