Fig. 6
β7-F185A KI mice are resistant to DSS-induced acute colitis. Eight-week-old WT, KI, and KO mice were treated with low concentration of DSS (2%) for 5 days, followed by regular drinking water (dosing). a–c Body weight change (a), survival ratio (b), and disease activity index (c) were obtained from individual groups of mice (n = 6 per group). d Quantitative PCR analysis of IL-6, TNF-α, and IL-1β expression in distal colon tissue from individual groups of mice (n = 6 per group) at day 10 after DSS treatment. Results are normalized to GAPDH. e Hematoxylin and eosin staining of distal colon sections at day 10 after DSS treatment. f Flow cytometric analysis of Treg cell number in colonic tissues from individual groups of mice (n = 6 per group) at day 4 after DSS treatment. g Immunofluorescence staining and quantification of F4/80+ macrophages and ICAM-1 expression in distal colon sections with anti-ICAM-1 (green), anti-F4/80 (red), and DAPI (blue) at day 4 after DSS treatment. Scale bars, 100 μm (e, g). **P < 0.005; ***P < 0.001; ns, not significant (two-way ANOVA in a, Student’s t test in c, d, f, and g). Data are mean ± s.d. of at least 3 independent experiments (a, c, d, f, g)